Fig. 6

Phenotyping of spheroids A Flow cytometry analysis of phenotype of spheroids Aire^WT or Aire^−/− at 12 h of adhesion by the expression of a viability marker and cell surface markers: UEA-1; Epcam; CD45; Ly51; MHCII and CD80 (representative figure from nine independent determinations). B Quantification of the markers by Prism GraphPad. Data shown (mean ± SD) are from nine independent determinations. The significant difference between AIRE ^WT and AIRE^−/− was analyzed by the unpaired t-test Epcam: **p = 0.0019; LY51: *p = 0.0471; UEA-1: *p = 0.0171; CD45: **p = 0.0069; MHCII: ns; CD80: *p = 0.0114. no significant difference