Fig. 9

Analysis of RNA sequencing data. A- Heat-map showing the large-scale expression profiling of differentially expressed mRNAs involved in cell adhesion pathway. Unsupervised heat-maps and dendrograms were constructed using the R platform. Heat-map legend: red = upregulated, blue = downregulated (Pearson’s correlation metrics, fold change ≥2.0 and false discovery rate (FDR) < 0.05). B Evaluation of the relative expression level of the mRNAs PLCB2, PARVB, P2RX7, VEGFC and ID1 by RT-qPCR. Difference between groups was analyzed by Unpaired-t test, comparing data from AIRE^WT vs. AIRE^−/− − p value = PLCB2 = ns 0,0738, PARVB = *0,0184, ID1 = ***0,0001, P2RX7 = ** 0,0089, VEGFC = *** 0,0002. C Genetic Interaction network. A network of 7 proteins identified with interaction with Aire. All nodes represent first order interaction. Colored edges convey status of predicted network edge correspondingly cyan, curated database; magenta, experimentally determined; forest green, gene neighborhood; red, gene fusion; navy blue, gene co-occurrence; lawn green, text mining; black, co-expression; lavender indigo, protein homology. Node color signifies protein functionality. Additional nodes are considered based on prediction score ≥ 0.9 (for more details, refer to STRING database)