Fig. 5

SDS-PAGE and western blot of membrane protein of OacB. Membrane proteins of mutants of OacB were prepared and subjected to western blot using anti-His antibodies. An equal amount of ~ 25 μg of each protein was loaded onto an SDS-PAGE gel. B2607 (wild type = WT) was used as positive control and B2372 was used as a negative control. Pre-stained PageRuler™ was used as a molecular mass marker. OacB protein band is indicated running at 35 kDa. (Membrane proteins tend to run differently onto SDS-PAGE from their actual molecular weight due to ‘gel shift’). B, Equal amounts of proteins loaded onto another SDS-PAGE gel and Coomassie-stained. C, Table summarizing location of critical residues in OacB. (Figure shows cropped images of the western blot and the Coomassie-stained gel. The uncropped full-length images of these are shown in Additional Fig. 3)