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Fig. 7 | BMC Molecular and Cell Biology

Fig. 7

From: Uncarboxylated osteocalcin promotes proliferation and metastasis of MDA-MB-231 cells through TGF-β/SMAD3 signaling pathway

Fig. 7

GluOC stimulates the growth and migration of MDA-MB-231 cells by activating the TGF-β/SMAD3 pathway. a After adding SIS3 and different concentrations of GluOC, we detected cyclin D1 and PCNA protein expression by western blotting. The results represent at least three independent experiments, and the data are presented as the mean ± SD (n = 3). *P < 0.05 and **P < 0.01, vs. the control group (con = control); $P < 0.05, $$P < 0.01 vs. the GluOC 40 ng/mL group; &P < 0.05, &&P < 0.01 vs. the GluOC 100 ng/mL group; #P < 0.05, ##P < 0.01 vs. the GluOC 160 ng/mL group. b After adding SIS3 and different concentrations of GluOC, we tested the migration capacity of MDA-MB-231 cells by Transwell assay. Photographs were taken with a microscope, with at least five field captured per treatment group, magnification × 100

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