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Fig. 1 | BMC Molecular and Cell Biology

Fig. 1

From: RNAP II produces capped 18S and 25S ribosomal RNAs resistant to 5′-monophosphate dependent processive 5′ to 3′ exonuclease in polymerase switched Saccharomyces cerevisiae

Fig. 1

Terminator resistant 18S and 25S rRNA molecules in Saccharomyces cerevisiae. SYBR-gold-stained gel (a) and Northern blot (b) showing rRNA extracted at different time points either treated (cut) or untreated (uncut) by Terminator. (c) Electropherograms used to validate the quality of RNA and to confirm the presence of Terminator resistant molecules. (d) Gel image generated from electropherograms by bioanalyzer software. (e) Terminator resistance percentage of ribosomal and nuclear RNA extracted from mid log (ML) and stationary (ST) wild type S. cerevisiae. (f) Relative HAT activity of various amounts of S. cerevisiae nuclear extract. Error bars represent standard deviation from three different experiments. Gel and membrane were cropped to show relevant information. Full length gel and membrane with visible edges are shown in Fig. S1

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