Fig. 5

MAPKAPK5-AS1 regulated ZEB1 expression through adsorbing miR-429. A The binding site between ZEB1 3’UTR and miR-429 was predicted via bioinformatics analysis. B The luciferase reporter assay showed that miR-429 could directly bind to ZEB1 3’UTR. C Spearman correlation analysis of ZEB1 mRNA expression and miR-429 expression in HCC tissues. D Western blot was used to detect the expression of ZEB1 in HepG2 and Huh7 cells after the transfection of si-MAPKAPK5-AS1 and si-MAPKAPK5-AS1/miR-429 inhibitors and in L02 cells transfected MAPKAPK5-AS1 overexpression plasmid and MAPKAPK5-AS1 overexpression plasmid/miR-429 mimics. E Lung metastasis assay in vivo was performed and the number of tumor nodules was counted. *P < 0.05; **P < 0.01