Fig. 4

CCT3 inhibits ferroptosis of LUAD cells. A shCtrl, shCCT3#1 and shCCT3#2 H2228 cells were subjected to PI/Annexin V-FITC staining and analyzed on flow cytometry. Left, representative images of Annexin V and PI positive cells. Right, quantification results. **p < 0.01. B and C Ctrl and CCT3 overexpressed H1975 cells treated without erastin (B) and with erastin for 8 h (C) were subjected to PI/Annexin V-FITC staining and analyzed on flow cytometry. Left, representative images of Annexin V and PI positive cells. Right, quantification results. There results in C showed statistical significance.**p < 0.01. D and E shCtrl, shCCT3#1 and shCCT3#2 H2228 cells (D), Ctrl and CCT3 overexpressed H1975 cells (E) were treated with DMSO, ferroptosis inducer erastin (5 uM) and apoptosis activator 2 (5 uM) in combination with or without ferroptosis inhibitor ferrostatin-1 (2 uM), apoptosis inhibitor Z-VAD-FMK (10 ug/ml) or 3-methylademine (2 mM). Then cell viability was examined by trypan blue staining. *p < 0.05. **p < 0.01