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Fig. 4 | BMC Molecular and Cell Biology

Fig. 4

From: Decreased Krüppel-like factor 4 in adenomyosis impairs decidualization by repressing autophagy in human endometrial stromal cells

Fig. 4

KLF4 directly induces ATG5 transcription. A and B hESCs (from fertile controls, n = 3) were infected with Ad-LacZ or Ad-His-KLF4 (MOI = 25 and 50) for 48 h. ATG5 mRNA and protein levels were measured by qRT–PCR and Western blotting, respectively. ***P < 0.001. C hESCs (from fertile controls, n = 3) were infected with Ad-LacZ or Ad-His-KLF4 (MOI = 50) for 48 h and then transfected with ATG5-Luc or ATG5-Luc deletion (300 ng/well). After 48 h, luciferase assays were performed, and the data were plotted after normalization to Renilla luciferase activity. **P < 0.01. D ChIP-PCR amplification using primers against the human ATG5 promoter region. PCR products were separated by agarose gel electrophoresis. Input (nonprecipitated) chromatin was utilized as a positive control in these analyses. E ABCD assays were performed using biotinylated or nonbiotinylated (competitor) double-stranded ATG5 wild-type (WT), conserved element-deleted (DEL) and conserved element-mutated (MUT) oligonucleotides with whole-cell extracts from hESCs infected with Ad-His-KLF4 or Ad-LacZ (MOI = 50) for 48 h

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