Table 1 Sequences of the primers for real-time PCR analysis
Gene | Primer sequence (5′ → 3′) |
|---|---|
VIM | forward: AATGACCGCTTCGCCAACT reverse: ATCTTATTCTGCTGCTCCAGGAA |
ZEB1 | forward: TGAGCAAAACCATGATCCTAATGT reverse: CAGGTGCCTCAGGAAAAATGA |
M-Sec | forward: TGCTCCAGAACCTGCATGAGGA reverse: AACTCAGGCAGCCTCGTGTCTA |
Cx43 | forward: GACAAGGTTCAAGCCTACTCAACTG reverse: TGTCCCCAGCAGCAGGAT |
ZO-1 | forward: CACGCAGTTACGAGCAAG reverse: TGAAGGTATCAGCGGAGG |
CTNNB1 | forward: AGCCGACACCAAGAAGCAGAGATG reverse: CGGCGCTGGGTATCCTGATGT |
IL1B | forward: ATGATGGCTTATTACAGTGGCAA reverse: GTCGGAGATTCGTAGCTGGA |
TLR2 | forward: GCTCGGAGTTCTCCCAGTTTC reverse: GAGCTGCCCTTGCAGATAC |
CCL22 | forward: ATTACGTCCGTTACCGTCTG reverse: TAGGCTCTTCATTGGCTCAG |
ACTB | forward: TGACGGGGTCACCCACACTGTGCCCATCTA reverse: CTAGAAGCATTTGCGGTGGACGATGGAGGG |