Fig. 1
From: Systematic study of single-cell isolation from musculoskeletal tissues for single-sell sequencing
Isolation of single cells from degenerated nucleus pulposus (NP) and Single-Cell RNA Sequencing (scRNA-seq). A. Lumber disc degeneration was shown on T2-weighted (T2-WI) Magnetic Resonance Imaging (MRI). The red circle marked the degenerated disc. B. Gross appearance of the degenerated NP obtained from surgery. C. Gross appearance of the cell suspension after 3–5 cycles of enzymatic treatment. Some white floccule residuals were labeled by black circle. D. Total cell numbers were counted automatically and manually, respectively. E. The isolated cell diameter distribution histogram. G. Histogram showing the cell output harvested via the rapid cycling enzymatic processing method (RAPID) or 4-h once-through digestion method (4-HOUR). H. Histogram showing proportion of live cells in each group. I. The live/dead immunofluorescence staining and quantitative analysis. J. Bioanalyzer trace of the scRNA-seq library showed the size distribution between 300 and 600 bp (blue arrow). H. The barcode-rank plot displayed gene expression counts after sequencing