From: Systematic study of single-cell isolation from musculoskeletal tissues for single-sell sequencing
Reference | Tissue | Species | Treatment | Number of profiling cells |
---|---|---|---|---|
Quanbo Ji, et al. [34] | Cartilage from 10 knee OA patients | Human | 0.25% Trypsin–EDTA for 30 min and then 0.2% type II collagenase (Sigma-Aldrich) for 4 h | 1600 |
Fiorella Carla Grandi, et al. [35] | OA samples from patients undergoing total joint replacement | Human | Collagenase II and IV (2.5Â mg/ml each; Worthington Biochem) overnight | Not reported |
Junxiang Li, et al. [36] | The distal cartilage structure at postnatal day 7 | Mouse | 0.2% collagenase II for 2Â h | 217 |
Vikram Sunkara, et al. [37] | Femur of 12Â weeks old mice | Mouse | 10Â mg/ml Collagenase II (Nordmark) supplemented for 4Â h | 7133 |
Koji Mizuhashi, et al. [38] | Femur growth plate cells at postnatal day 2 | Mouse | Liberase TM (Sigma/Roche) for unclear time | 18,000 |
Natalie H Kelly, et al. [39] | Hindlimb bud of embryonic mice | Mouse | Collagenase Type II (Worthington-biochem, Lakewood, NJ) and pronase (EMD Millipore, Billerica, MA) in 15Â min increments with agitation for up to 1Â h | 9466 |