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Table 2 Summary of single-cell isolation protocols for cartilage in reported studies using scRNA-seq technique

From: Systematic study of single-cell isolation from musculoskeletal tissues for single-sell sequencing

Reference

Tissue

Species

Treatment

Number of profiling cells

Quanbo Ji, et al. [34]

Cartilage from 10 knee OA patients

Human

0.25% Trypsin–EDTA for 30 min and then 0.2% type II collagenase (Sigma-Aldrich) for 4 h

1600

Fiorella Carla Grandi, et al. [35]

OA samples from patients undergoing total joint replacement

Human

Collagenase II and IV (2.5 mg/ml each; Worthington Biochem) overnight

Not reported

Junxiang Li, et al. [36]

The distal cartilage structure at postnatal day 7

Mouse

0.2% collagenase II for 2 h

217

Vikram Sunkara, et al. [37]

Femur of 12 weeks old mice

Mouse

10 mg/ml Collagenase II (Nordmark) supplemented for 4 h

7133

Koji Mizuhashi, et al. [38]

Femur growth plate cells at postnatal day 2

Mouse

Liberase TM (Sigma/Roche) for unclear time

18,000

Natalie H Kelly, et al. [39]

Hindlimb bud of embryonic mice

Mouse

Collagenase Type II (Worthington-biochem, Lakewood, NJ) and pronase (EMD Millipore, Billerica, MA) in 15 min increments with agitation for up to 1 h

9466