Fig. 2

KLF5 overexpression mediates the proliferation, migration, and invasion of nasopharyngeal carcinoma (NPC) cells. a Western blot analysis indicating the protein expression of KLF5, E-cadherin and N-cadherin in NP69, HONE-1, NPC-TW01, NPC-TW03, and NPC-TW04 cells, were probes with anti KLF5, E-cadherin and N-cadherin and specific antibodies for each compartments corresponding to anti β-actin. b Real-time PCR results showing the mRNA expression of KLF5 in NP69 and NPC cell lines using GAPDH as a reference gene. c Western blot analysis indicating the expression of KLF5 in NPC-TW03 and NPC-TW04 cells after CMV or CMV-KLF5 vector transfection, were probes with anti KLF5 and specific antibodies for each compartments corresponding to anti β-actin. d Proliferation rates of NPC cells 24 to 72 h post-transfection with CMV or CMV-KLF5. e Proliferation rates of NPC cells at 72 h post-transfection with CMV or CMV-KLF5 was analyzed by BrdU ELISA cell proliferation assay. f NPC-TW03 and NPC-TW04 cells were transiently transfected with CMV or CMV-KLF5 and analyzed by wound healing assays. g NPC-TW03 and NPC-TW04 cells were transiently transfected with CMV and CMV-KLF5 and analyzed by invasion assays. Data are shown as mean ± SD from three independent experiments. *P < 0.05, **P < 0.01, respectively. N.S., not significant