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Fig. 5 | BMC Molecular and Cell Biology

Fig. 5

From: MicroRNA-145-5p modulates Krüppel-like factor 5 and inhibits cell proliferation, migration, and invasion in nasopharyngeal carcinoma

Fig. 5

MiR-145-5p and KLF5 modulates phospho-FAKTyr397 expression in nasopharyngeal carcinoma (NPC) cells. a MiR-145-5p induced p21 and reduced cyclin D1, cyclin B1, pro-caspase 3 and PARP protein expression post-transfection with scramble control or miR-145-5p mimics in NPC-TW03 and NPC-TW04 cells, were probes with anti cyclin D1, cyclin B1, p21, pro-caspase 3, PARP and specific antibodies for each compartments corresponding to anti α-tubulin. b MiR-145-5p down-regulates phospho-FAKTyr397 protein expression post-transfection with scramble control or miR-145-5p mimics in NPC-TW03 and NPC-TW04 cells, were probes with anti phospho-FAKTyr397, FAK and specific antibodies for each compartments corresponding to anti β-actin. c Quantification of phospho-FAKTyr397/FAK expression are the average of three independent experiments, using the Image J software and β-actin as a reference. d Western blot analysis indicating phospho-FAKTyr397 and FAK expression in NPC-TW03 and NPC-TW04 cells post-transfection with CMV or CMV-KLF5 vectors, were probes with anti phospho-FAKTyr397, FAK and specific antibodies for each compartments corresponding to anti β-actin. e Quantification of phospho-FAKTyr397/FAK expression are the average of three independent experiments, using the Image J software and β-actin as a reference. f The possible molecular network between KLF5 and FAK was analyzed by Ingenuity Pathway Analysis. g PROMO tool identified the putative transcription factor binding sites in FAK DNA sequences. Data are shown as mean ± SD from three independent experiments. *P < 0.05, **P < 0.01, respectively

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