Table 4 The impact of mutation of interacting residues on p37-p97 binding affinity
The effect of mutation on binding affinity | ||
|---|---|---|
Interacting residues of p37 UBX domain | Predicted affinity change (ΔΔG affinity) | Binding affinity |
PHE(304)-ALA | -0.732Â kcal/mol | Decreasing affinity |
LEU(269)-ALA | -0.267Â kcal/mol | Decreasing affinity |
ASN(306)-ALA | -0.446Â kcal/mol | Decreasing affinity |
LEU(322)-ALA | -0.683Â kcal/mol | Decreasing affinity |
GLN(260)-ALA | -0.897Â kcal/mol | Decreasing affinity |
VAL(325)-ALA | -0.582Â kcal/mol | Decreasing affinity |
ARG(262)-ALA | -1.147Â kcal/mol | Decreasing affinity |