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Fig. 2 | BMC Molecular and Cell Biology

Fig. 2

From: Activated TRPA1 plays a therapeutic role in TMZ resistance in glioblastoma by altering mitochondrial dynamics

Fig. 2

Effect of TMZ and a TRPA1 agonist and inhibitor on the apoptosis rate. PF-4840154 and TMZ cotreatment increased the activity of Caspase-3 and Caspase-9, and the protein expression of Cleaved Caspase-3 (A, B) in U251 cells compared to the control group and TMZ group. In the SHG-44 cells, the TMZ group and PF-4840154 and TMZ cotreatment group showed increased Caspase-3 activity and Cleaved Caspase-3 protein expression, and the HC030031 and TMZ cotreatment group showed decreased Cleaved Caspase-3 protein expression (E). PF-4840154 and TMZ cotreatment increased the activity of Caspase-3 and Caspase-9 (D) in the SHG-44 cells compared to the control and TMZ group. Western blotting was performed to detect the levels of Bcl-2 protein, BAX protein and MGMT protein after TMZ or drug treatment in U251 cells (C) and SHG-44 cells (F). GAPDH was used as an internal reference. Cropped photos of the blots are displayed, and the expression level was quantified by densitometry with ImageJ software. *P < 0.05, **P < 0.01, ***P < 0.001,****P < 0.0001

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