Fig. 4

CLCA2 inhibits ocervical cancer growth and EMT in vivo. A, B CLCA2 overexpression led to a obvious reduction in tumor volume. C HE staining showed that the tumor cells had nuclear pyknosis and apoptosis. D TUNEL staining showed TUNEL positive cells were observed in tumors from CLCA2 overexpression treatment than those from NC treatment. E Immunofluorescence analysis of E-cadherin, N-cadherin, snail, twist and p-p38. F Westernblot analysis of E-cadherin, N-cadherin, snail, twist and p38. Scale bar = 100 μm, *P < 0.05, **P < 0.001, t-test. Scale bar = 25um