Fig. 2

Up-regulated expression of RPL5 in COAD. A Data from TCGA database demonstrated a significant upregulation of RPL5 expression in COAD tumor samples (n = 290) compared with normal tissue (n = 41). B Data from GTEx database and TCGA tumor tissue demonstrated a significant upregulation of RPL5 expression in COAD tumor samples (n = 290) compared with normal tissue (n = 349). C Nine pairs of cancer tissues and paired adjacent tissues from clinical colon cancer patients were collected, and the differential expression of RPL5 in colon cancer tissues and paired adjacent tissues was detected by western blotting. The blots were cut prior to hybridization with antibodies during blotting. D The proteins of human normal colon epithelial cells NCM460 and colon cancer cells HCT116, HT29, RKO, SW480 and KM12 were extracted, and the expression of RPL5 in colon cancer was detected by western blotting. The blots were cut prior to hybridization with antibodies during blotting. E The TCGA database was used to analyze the correlation between the expression of RPL5 and the stage of clinical colon cancer patients