Fig. 2

ARNTL2 potentiates the transcription activity of ACOT7 via direct interaction to its promoter sequence. A RT-qPCR and immunoblotting analysis of ARNTL2 in H1299 cells transfected with Ctrl and ACOT7 overexpressing lentivirus. Quantification of immunoblotting results was presented. **p < 0.01. B RT-qPCR and immunoblotting analysis of ACOT7 in A549 cells transfected with siNC, siARNTL2#1 and siARNTL2#2. Quantification of immunoblotting results was presented. *p < 0.05. C Dual luciferase activity of ACOT7 promoter was assessed in H1299 cells with ARNTL2 overexpressiong and in A549 cells with ARNTL2 knockdown. *p < 0.05. D Immunoblotting analysis of ARNTL2 in H1299 and A549 cells transfected with pCDNA3.1-Flag (Flag) and pCDNA3.1-ARNTL2-Flag (ARNTL2-Flag). Chip-qPCR was performed to determine the binding of ARNTL2 to the promoter sequence of ACOT7. ***p < 0.001. E Sperman correlation between ARNTL2 and ACOT7 in LUAD patients based on TCGA database. p < 0.0001. F Overall survival was analyzed for LUAD patients who were divided into ACOT7 high and low expression group. n = 335 per group. p = 0.00096. The blots were cut prior to hybridisation with antibodies during immunoblotting experiments