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Fig. 3 | BMC Molecular and Cell Biology

Fig. 3

From: ARNTL2 upregulation of ACOT7 promotes NSCLC cell proliferation through inhibition of apoptosis and ferroptosis

Fig. 3

ACOT7 promotes the growth and proliferation of NSCLC cells. A RT-qPCR and immunoblotting analysis of ACOT7 in Beas-2B, A549, H1975 and H1299 cells. *p < 0.05. **p < 0.01. B RT-qPCR and immunoblotting analysis of ACOT7 in siNC, siACOT7#1 and siACOT7#2 A549 cells. **p < 0.01. C and D Cell proliferation was tested by CCK8 (C) and colony formation (D) assay in siNC, siACOT7#1 and siACOT7#2 A549 cells. **p < 0.01. E RT-qPCR and immunoblotting analysis of ACOT7 in H1299 cells transfected with Ctrl and ACOT7 overexpression lentivirus. **p < 0.01. F and G Cell proliferation was tested by CCK8 (F) and colony formation (G) assay in H1299 cells transfected with Ctrl and ACOT7 overexpression lentivirus. *p < 0.05. **p < 0.01. The blots were cut prior to hybridisation with antibodies during immunoblotting experiments

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