Fig. 5

Cytoplasm HMGB1 mediates LPS-induced autophagy of macrophages. A. Macrophage RAW264.7 were pretreated with 25 mmol/l anacardic acid (AA) and then treated with LPS (1 μg/ml) or LPS combined anti-HMGB1 antibodies (10 μg/ml) for 12 h. HMGB1 expression and location were detected by immunofluorescence. B, C. Macrophage RAW264.7 were pretreated with AA and then treated with LPS or LPS combined HMGB1 (500 ng/ml) or anti-HMGB1 antibodies for 12 h. LC3 expression were detected by western blot. **P < 0.01 versus the control group. ^##P < 0.01 versus the LPS group. ^&&P < 0.01 versus the LPS + AA + HMGB1 group