Fig. 6
From: HMGB1 mediates lipopolysaccharide-induced macrophage autophagy and pyroptosis
Extracellular HMGB1 up-regulates pyroptosis-related proteins levels in macrophage. Macrophage RAW264.7 were pretreated with AA and then treated with LPS or LPS combined HMGB1 or anti-HMGB1 antibodies for 36 h. A, B. The pyroptosis-related protein expressions were detected by western blot. C. The levels of caspase-1 activity were detected. D. The rate of pyroptotic cell was detected by flow cytometry. *P < 0.05, **P < 0.01 versus the control group. #P < 0.05, ##P < 0.01 versus the LPS group. $P < 0.05, $$P < 0.01 versus the LPS + AA group. &P < 0.05, &&P < 0.01 versus the LPS + AA + HMGB1 group