Fig. 2

Effect of ribosomal protein L11 (RPL11) on cell cycle progression in non-small cell lung cancer (NSCLC) cells. Distribution of the cell cycle stages in NSCLC cells after overexpression (a) or siRNA-mediated knockdown (b) of RPL11 was assessed by flow cytometry. The levels of cell cycle-related proteins cyclin D1 (CCND1) and cyclin-dependent kinase (CDK4) in RPL11 overexpressed (c) or siRNA-transfected (d) groups were detected by western blotting. The blots were cut before hybridization with antibodies during blotting (c, d). The data are presented as mean ± SD from three independent trials (n = 3). Statastic data of a and c was analyzed using unpaired student’s t-test, statistic data of b and d was analyzed using one-way ANOVA. *P < 0.05, **P < 0.01, ***P < 0.001