Fig. 3

Effect of ribosomal protein L11 (RPL11) on autophagy in non-small cell lung cancer (NSCLC) cells. (a) Effect of RPL11 on autolysosome and autophagosome formation in NSCLC cells: cells transfected with RPL11 vector or its control vector GV141 (vector) were infected with mRFP-GFP-LC3 containing adenovirus: 48 h later, the cells were photographed using a laser confocal microscopy. (b) Effect of RPL11 overexpression or siRNA knockdown on autophagy-related proteins (LC3, BECN1 and p62) expression were detected by western blotting. (c-e) Effect of autophagy inhibitor chloroquine (CQ) on cell growth in RPL11-overexpressed NSCLC cells determined using the CCK-8 (c), clone formation (d), and flow cytometry (e) assays. (f) Expression levels of autophagy and G0/G1 phase-related proteins in RPL11 or its control vector transfected cells, and with the addition of 20 µM CQ or an equal volume of CQ solvent, were examined using western blotting. Blots of b and f were cut before hybridization with antibodies during blotting. The data are presented as mean ± SD from three independent trials (n = 3). Protein expression comparison between RPL11 overexpression group (RPL11) and control vector group (Vector) in b was analyzed using Student’s t-test. Statistical data of c, e, f, and comparison of proteins expression in si-nc and siRPL11 groups (b) was analyzed using one-way ANOVA. *P < 0.05, **P < 0.01, ***P < 0.001