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Fig. 4 | BMC Molecular and Cell Biology

Fig. 4

From: Cyclic tensile force modifies calvarial osteoblast function via the interplay between ERK1/2 and STAT3

Fig. 4

The osteoblast differentiation and mineralization of primary osteoblasts after STAT3 inhibition in the presence or absence of tensile loading. Osteoblasts were treated with Stattic for 6 h, followed by mechanical loading for 24 h (A) The mRNA expressions of COL-I, ALP, and Runx2 were detected by qPCR. (B) Western blots analysis of the osteogenic markers COL-I, ALP, and Runx2. (C) Alizarin red staining showed the formation of mineralized nodules of osteoblasts after STAT3 inhibition and (D) ALP activity (scale bar: 200 μm). Bars represent mean ± SD. *p < 0.05

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BMC Molecular and Cell Biology

ISSN: 2661-8850

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