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Fig. 5 | BMC Molecular and Cell Biology

Fig. 5

From: Cyclic tensile force modifies calvarial osteoblast function via the interplay between ERK1/2 and STAT3

Fig. 5

ERK1/2 and STAT3 had interaction and their activation influenced each other. (A) Representative images of Co-IP. (B) Immunofluorescence assay showed the subcellular location of ERK1/2 and STAT3 was affected by loading, U0126, and Stattic administration. (C) Western blot analysis of STAT3 and pSTAT3 protein expression in whole cell lysates. (D) Western blot analysis of EKR1/2 and pERK1/2 protein expression in whole cell lysates. (E) Western blot analysis was performed to examine the protein levels of pERK1/2 and pSTAT3 in nuclear fractions. Bars represent mean ± SD. *p < 0.05

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