Table 1 Primer sequences and product sizes for quantitative polymerase chain reaction
Target gene | Sequences (5’-3’)* | Product sizes (bp) |
|---|---|---|
Runx2 | F: ATCTACACCGTGGGATATTCCA R: GCAGCATAAACGACAGGAACA | 130 |
Osterix | F: CCTCTTGAGAGGAGACGGGA R: GGGCTGAAAGGTCAGCGTAT | 157 |
OCN | F: GCACACCTAGCAGACACCAT R: GTGTTGTCCCTTCCCCTCTG | 135 |
COL-I | F: GATGTGGAATACGAACTGGATG R: TGGGAATGCTTGTGTCTGG | 134 |
ALP | F: GAACAGACCCTCCCCACGAG R: CCTGAGATTCGTCCCTCGC | 104 |
GAPDH | F: ACAGCAACAGGGTGGTGGAC R: TTTGAGGGTGCAGCGAACTT | 136 |