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Fig. 1 | BMC Molecular and Cell Biology

Fig. 1

From: Role of the human solute carrier family 14 member 1 gene in hypoxia-induced renal cell carcinoma occurrence and its enlightenment to cancer nursing

Fig. 1

Effect of hypoxia/normoxia on the biological characteristics of A498 cells. A498 cells were cultured at 37 °C in a humidified incubator with 5% CO2, 2% O2, and 93% N2 (hypoxia) or with 5% CO2 and 95% O2 (normoxia) for 24 and 48 h. a The invasion of A498 cells was detected by the transwell assay. b The migration of A498 cells was examined by the wound healing assay. c The apoptosis of A498 cells was measured by flow cytometry. d The viability of A498 cells was assessed by the CCK-8 assay. e Expression of the UT-B protein was detected by western blot. Full-length blots/gels are presented in Supplementary file 5. The levels of IL-6 (f) and TNF-α (g) in the culture supernatant were determined by a Beckman Coulter AU5800 automatic biochemical analyzer. Each bar represents the median expression obtained from 3 independent experiments. *P < 0.05, **P < 0.01 vs the corresponding normoxic group

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