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Fig. 3 | BMC Molecular and Cell Biology

Fig. 3

From: Role of the human solute carrier family 14 member 1 gene in hypoxia-induced renal cell carcinoma occurrence and its enlightenment to cancer nursing

Fig. 3

Effect of SLC14A1 overexpression on mitochondrial dysfunction in A498 cells. A498 cells were transfected with either the pc-SLC14A1 vector (2.5 μg/ml) or pcDNA3.1 null vector (pc-NC, 2.5 μg/ml) for 48 h. a The fluorescent probe MitoSOX™ Red was used to assess mitochondrial ROS level. b The JC-1 probe was used to determine changes in the mitochondrial membrane potential. c Observation of A498 cell morphology was performed using electron microscopy (× 5,200). Red arrows indicate abnormal mitochondria structure, including mitochondrial swelling and rupture. Scale bar = 1 μm. d The ATP level of A498 cells was evaluated using a luciferase-based assay kit. Results are presented as the mean ± SD from 3 independent experiments. **P < 0.01 vs the pc-NC group

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