Skip to main content
Fig. 4 | BMC Molecular and Cell Biology

Fig. 4

From: Role of the human solute carrier family 14 member 1 gene in hypoxia-induced renal cell carcinoma occurrence and its enlightenment to cancer nursing

Fig. 4

Effect of SLC14A1 overexpression on hypoxia-induced biological characteristics of A498 cells. A498 cells were transfected with either the pc-SLC14A1 vector (2.5 μg/ml) or pcDNA3.1 null vector (pc-NC, 2.5 μg/ml) and then cultured at 37 °C in a humidified incubator with 5% CO2, 2% O2, and 93% N2 (hypoxia) for 48 h. a The apoptosis of A498 cells was measured by flow cytometry. b The distribution of the cell cycle was calculated by flow cytometry. (c) The invasion of A498 cells was assessed by the transwell assay. d The migration of A498 cells was analyzed by the wound healing assay. The levels of IL-6 e and TNF-α f in the cell culture supernatant were measured by the Beckman Coulter AU5800 automatic biochemical analyzer. n = 3, **P < 0.01, *P < 0.05, #P > 0.05 versus the hypoxia-alone group

Back to article page