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Fig. 5 | BMC Molecular and Cell Biology

Fig. 5

From: Using RNA-seq to identify suitable housekeeping genes for hypoxia studies in human adipose-derived stem cells

Fig. 5

Comparative study of published methods for quantitating variance in normalised Ct values of housekeeping genes in hADSC under Nx and Hx culture conditions. Raw Ct values of the 5 HKG in hADSC under Nx and Hx conditions were normalised and ranked using A) GeNorm (geometric mean), B) NormFinder (model-based variance estimation), C) BestKeeper (pair-wise correlations) D) comparative delta-Ct, E) RefFinder (geometric mean of each ranking), F) relative expression calculated with 2−△△Ct (Livak and Schmittgen 2001) using all HKG candidates as reference genes in a step-wise manner to compare between Hx and Nx, and G) The final ranking of all HKG candidates in each hADSC

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