Fig. 2

IL-6 decreases IL-1β and IFN-γ induced p-eIF2-α and p-IRE1 expression in INS-1E cells. Cells were exposed to IL-6 (IL6) or untreated (Ctrl). After 24 h cells were exposed to IL-1β and IFN-γ in the absence (Cyto) or in the presence of pre-treatment with IL-6 (IL6 + Cyto) or continuous treatment of IL-6 (IL6 + IL6 + Cyto). A Western blot for p-eIF2-α and α-Tubulin (note that α-Tubulin image is the same used in Fig. 1A, since they are from the same blot where caspase 3 and eiF2α were evaluated). B The barplot showing the mean values obtained in five independent experiments corrected by the housekeeping protein α-Tubulin. One-way ANOVA followed by Sidak’s correction. *p < 0,05 vs Ctrl. C Western blot for p-IRE1 and α-Tubulin. D The barplot showing the mean values obtained in four independent experiments corrected by the housekeeping protein α-Tubulin