Fig. 4

Superimposed structures of native CYP4F2 (black color) and CYP4F2*3 (V433M) variant (red color), the position of wild-type residue (V433) and mutant-type residue (M433) was characterized. The close-up view of V433 location illustrates that mutation to M433 caused changes from helix structure to turn which resulted in more flexibility in 445–460 region in mutant form compared to native. V433 shows in green stick and M433 shows in blue stick form. The 445–470 region is colored in yellow and cyan in mutant and native form, respectively