Articles

Lamin A/C and Emerin depletion impacts chromatin organization and dynamics in the interphase nucleus

Nuclear lamins are type V intermediate filament proteins that maintain nuclear structure and function. Furthermore, Emerin - an interactor of Lamin A/C, facilitates crosstalk between the cytoskeleton and the n...

Authors: Devika Ranade, Roopali Pradhan, Muhunden Jayakrishnan, Sushmitha Hegde and Kundan Sengupta

High resolution crystal structure of the FAK FERM domain reveals new insights on the Druggability of tyrosine 397 and the Src SH3 binding site

Focal Adhesion Kinase (FAK) is a major cancer drug target that is involved in numerous aspects of tumor progression and survival. While multiple research groups have developed ATP-competitive small molecule in...

Authors: Timothy Marlowe, Alexey Dementiev, Sheila Figel, Andrew Rivera, Michael Flavin and William Cance

Downregulation of lncRNA CCAT1 enhances 5-fluorouracil sensitivity in human colon cancer cells

The purpose of this study was to determine the aberrant expression of the long noncoding RNA (lncRNA) colon cancer-associated transcript 1 (CCAT1) in 5-fluorouracil-resistant colonic neoplasm cells and to eluc...

Authors: Chun Yang, Yong Pan and Shao Ping Deng

The role of Patronin in Drosophila mitosis

The calmodulin-regulated spectrin-associated proteins (CAMSAPs) belong to a conserved protein family, which includes members that bind the polymerizing mcrotubule (MT) minus ends and remain associated with the...

Authors: Gera A. Pavlova, Alyona V. Razuvaeva, Julia V. Popova, Evgeniya N. Andreyeva, Lyubov A. Yarinich, Mikhail O. Lebedev, Claudia Pellacani, Silvia Bonaccorsi, Maria Patrizia Somma, Maurizio Gatti and Alexey V. Pindyurin

Dlx5 and Dlx6 can antagonize cell division at the G₁/S checkpoint

Dlx5 and Dlx6 stimulate differentiation of diverse progenitors during embryonic development. Their actions as pro-differentiation transcription factors includes the up-regulation of differentiation markers but th...

Authors: Rachel K. MacKenzie, Parvathy Ravi Sankar and Andrew J. Bendall

Compression and tension variably alter Osteoprotegerin expression via miR-3198 in periodontal ligament cells

Osteoclasts play a critical role in bone resorption due to orthodontic tooth movement (OTM). In OTM, a force is exerted on the tooth, creating compression of the periodontal ligament (PDL) on one side of the t...

Authors: Hiroyuki Kanzaki, Satoshi Wada, Yuuki Yamaguchi, Yuta Katsumata, Kanako Itohiya, Sari Fukaya, Yutaka Miyamoto, Tsuyoshi Narimiya, Koji Noda and Yoshiki Nakamura

Short telomeres correlate with a strong induction of cellular senescence in human dental follicle cells

Dental follicle cells (DFCs) are dental stem cells and interesting options for regenerative therapies in dentistry. However, DFCs acquire replicative senescence in long-term cultures, but little is known about...

Authors: Christian Morsczeck, Anja Reck and Torsten E. Reichert

Highly efficient correction of structural mutations of 450 kb KIT locus in kidney cells of Yorkshire pig by CRISPR/Cas9

The white coat colour of Yorkshire and Landrace pig breeds is caused by the dominant white I allele of KIT, associated with 450-kb duplications and a splice mutation (G > A) at the first base in intron 17. To tes...

Authors: Ke Qin, Xinyu Liang, Guanjie Sun, Xuan Shi, Min Wang, Hongbo Liu, Yaosheng Chen, Xiaohong Liu and Zuyong He

Genes involved in mitochondrial biogenesis and function may not show synchronised responses to mitochondria in shell gland of laying chickens under infectious bronchitis virus challenge

Egg formation takes place in the oviduct of laying hens over a 24 h period. Infectious bronchitis virus (IBV) causes pathological lesions in the chicken oviduct. In the current study, mitochondrial counts were...

Authors: Samiullah Khan, Juliet Roberts and Shu-Biao Wu

Generation and maintenance of acentric stable double minutes from chromosome arms in inter-species hybrid cells

Extrachromosomal acentric double minutes (DMs) contribute to human malignancy by carrying amplified oncogenes. Recent cancer genomics revealed that the pulverization of defined chromosome arms (chromothripsis)...

Authors: Noriaki Shimizu, Rita Kapoor, Shuhei Naniwa, Naoto Sakamaru, Taku Yamada, You-ki Yamamura and Koh-ichi Utani

Fission yeast type 2 node proteins Blt1p and Gef2p cooperate to ensure timely completion of cytokinesis

The conserved NDR-family kinase Sid2p localizes to the contractile ring during fission yeast cytokinesis to promote ring constriction, septation, and completion of cell division. Previous studies have found th...

Authors: Lois Kwon, Emma M. Magee, Alexis Crayton and John W. Goss

Mitotic activity patterns and cytoskeletal changes throughout the progression of diapause developmental program in Daphnia

Diapause is a form of dormancy that is genetically predetermined to allow animals to overcome harsh environmental conditions. It is induced by predictive environmental cues bringing cellular activity levels in...

Authors: Luxi Chen, Rosemary E. Barnett, Martin Horstmann, Verena Bamberger, Lea Heberle, Nina Krebs, John K. Colbourne, Rocío Gómez and Linda C. Weiss

Shikonin sensitizes A549 cells to TRAIL-induced apoptosis through the JNK, STAT3 and AKT pathways

TRAIL, tumor necrosis factor-related apoptosis-inducing ligand, can selectively kill cancer cells with little or no cytotoxicity toward normal human cells and is regarded as a potential relatively safe antitum...

Authors: Zhi Lan Guo, Jing Zhe Li, Yan Yan Ma, Dan Qian, Ju Ying Zhong, Meng Meng Jin, Peng Huang, Lu Yang Che, Bing Pan, Yi Wang, Zhen Xiao Sun and Chang Zhen Liu

Post-treatment de-phosphorylation of p53 correlates with dasatinib responsiveness in malignant melanoma

Dasatinib (Sprycel) was developed as a tyrosine kinase inhibitor targeting Bcr-Abl and the family of Src kinases. Dasatinib is commonly used for the treatment of acute lymphoblastic and chronic myelogenous leu...

Authors: Josip Skoko, Jan Rožanc, Emilie M. Charles, Leonidas G. Alexopoulos and Markus Rehm

Comparative evaluation of mesenchymal stromal cells from umbilical cord and amniotic membrane in xeno-free conditions

Within the past years, umbilical cord (UC) and amniotic membrane (AM) expanded in human platelet lysate (PL) have been found to become increasingly candidate of mesenchymal stromal cells (MSCs) in preclinical ...

Authors: Yongxu Mu, Xiaoyun Wu and Zhiming Hao

The STRIPAK complex components FAM40A and FAM40B regulate endothelial cell contractility via ROCKs

Endothelial cells provide a barrier between blood and tissues, which is regulated to allow molecules and cells in out of tissues. Patients with cerebral cavernous malformations (CCM) have dilated leaky blood v...

Authors: Narendra Suryavanshi, Joanna Furmston and Anne J. Ridley

Kif17 phosphorylation regulates photoreceptor outer segment turnover

KIF17, a kinesin-2 motor that functions in intraflagellar transport, can regulate the onset of photoreceptor outer segment development. However, the function of KIF17 in a mature photoreceptor remains unclear....

Authors: Tylor R. Lewis, Sean R. Kundinger, Brian A. Link, Christine Insinna and Joseph C. Besharse

PIAS1 protects against myocardial ischemia-reperfusion injury by stimulating PPARγ SUMOylation

Myocardial ischemia-reperfusion injury (IRI) has become one of the most serious complications after reperfusion therapy in patients with acute myocardial infarction. Small ubiquitin-like modification (SUMOylat...

Authors: Bo Xie, Xinyu Liu, Jie Yang, Jinke Cheng, Jianmin Gu and Song Xue

Inflammation caused by peripheral immune cells across into injured mouse blood brain barrier can worsen postoperative cognitive dysfunction induced by isoflurane

Disruption to the blood brain barrier (BBB) is a leading factor associated with the development of postoperative cognitive dysfunction (POCD). Despite this, the underlying mechanism by which BBB disruption pro...

Authors: Honghua Zhu, Wei Liu and Hao Fang

Acetylation of C-terminal lysines modulates protein turnover and stability of Connexin-32

The gap junction protein, Connexin32 (Cx32), is expressed in various tissues including liver, exocrine pancreas, gastrointestinal epithelium, and the glia of the central and peripheral nervous system. Gap junc...

Authors: Sarah R. Alaei, Charles K. Abrams, J. Chloë Bulinski, Elliot L. Hertzberg and Mona M. Freidin

Tanshinone IIA reduces SW837 colorectal cancer cell viability via the promotion of mitochondrial fission by activating JNK-Mff signaling pathways

Mitochondrial homeostasis has been increasingly viewed as a potential target of cancer therapy, and mitochondrial fission is a novel regulator of mitochondrial function and apoptosis. The aim of our study was ...

Authors: Sayilaxi Jieensinue, Hong Zhu, Guangcheng Li, Keli Dong, Meiting Liang and Yayue Li

Correction to: The M-phase specific hyperphosphorylation of Staufen2 involved the cyclin-dependent kinase CDK1

Following publication of the original article [1], the authors reported a change to one of the author names.

Authors: Rémy Beaujois, Elizabeth Ottoni, Xin Zhang, Christina Gagnon, Sami Hassine, Stéphanie Mollet, Wildriss Viranaicken and Luc DesGroseillers

Treatment of Trypanosoma cruzi with 2-bromopalmitate alters morphology, endocytosis, differentiation and infectivity

The palmitate analogue 2-bromopalmitate (2-BP) is a non-selective membrane tethered cysteine alkylator of many membrane-associated enzymes that in the last years emerged as a general inhibitor of protein S-pal...

Authors: Cassiano Martin Batista, Rafael Luis Kessler, Iriane Eger and Maurilio José Soares

Lipopolysaccharide induces bacterial autophagy in epithelial keratinocytes of the gingival sulcus

Interactions of resident bacteria and/or their producing lipopolysaccharide (LPS) with sulcular epithelial keratinocytes may be regulated by autophagy in the gingival sulcus. In this study, we investigated an ...

Authors: Kanako Hagio-Izaki, Madoka Yasunaga, Masahiro Yamaguchi, Hiroshi Kajiya, Hiromitsu Morita, Masahiro Yoneda, Takao Hirofuji and Jun Ohno

Fibroblasts from bank voles inhabiting Chernobyl have increased resistance against oxidative and DNA stresses

Elevated levels of environmental ionizing radiation can be a selective pressure for wildlife by producing reactive oxygen species and DNA damage. However, the underlying molecular mechanisms that are affected ...

Authors: Venla Mustonen, Jenni Kesäniemi, Anton Lavrinienko, Eugene Tukalenko, Tapio Mappes, Phillip C. Watts and Jaana Jurvansuu

Mutant TP53 G245C and R273H promote cellular malignancy in esophageal squamous cell carcinoma

TP53 gene mutations occur in more than 50% of human cancers and the vast majority of these mutations in human cancers are missense mutations, which broadly occur in DNA binding domain (DBD) (Amino acids 102–292) ...

Authors: Nan Kang, Yu Wang, Shichao Guo, Yunwei Ou, Guangchao Wang, Jie Chen, Dan Li and Qimin Zhan

Generation of renewable mouse intestinal epithelial cell monolayers and organoids for functional analyses

Conditional reprogramming has enabled the development of long-lived, normal epithelial cell lines from mice and humans by in vitro culture with ROCK inhibitor on a feeder layer. We applied this technology to m...

Authors: Emily C. Moorefield, R. Eric Blue, Nancy L. Quinney, Martina Gentzsch and Shengli Ding

Novel roles for scleraxis in regulating adult tenocyte function

Tendinopathies are common and difficult to resolve due to the formation of scar tissue that reduces the mechanical integrity of the tissue, leading to frequent reinjury. Tenocytes respond to both excessive loa...

Authors: Anne E. C. Nichols, Robert E. Settlage, Stephen R. Werre and Linda A. Dahlgren

Cyclic AMP signaling in Dictyostelium promotes the translocation of the copine family of calcium-binding proteins to the plasma membrane

Copines are calcium-dependent phospholipid-binding proteins found in many eukaryotic organisms and are thought to be involved in signaling pathways that regulate a wide variety of cellular processes. Copines a...

Authors: April N. Ilacqua, Janet E. Price, Bria N. Graham, Matthew J. Buccilli, Dexter R. McKellar and Cynthia K. Damer

mTOR and ROS regulation by anethole on adipogenic differentiation in human mesenchymal stem cells

Adipocyte differentiation of human mesenchymal stem cells (hMSCs) is dependent on mitochondrial metabolism and reactive oxygen species (ROS) to initiate adipocyte differentiation. Although anethole has been kn...

Authors: Yun-Hee Rhee, Jeong Hwan Moon, Ji-Hun Mo, Tiffany Pham and Phil-Sang Chung

Caspase-independent cell death does not elicit a proliferative response in melanoma cancer cells

Apoptosis, the most well-known type of programmed cell death, can induce in a paracrine manner a proliferative response in neighboring surviving cells called apoptosis-induced proliferation (AiP). While having...

Authors: Ahlima Roumane, Kevin Berthenet, Chaïmaa El Fassi and Gabriel Ichim

Establishment and comparison of air-liquid interface culture systems for primary and immortalized swine tracheal epithelial cells

Air-liquid interface (Ali) systems allow the establishment of a culture environment more representative of that in vivo than other culture systems. They are useful for performing mechanistic studies of respira...

Authors: Haiyan Wang, Lina He, Beibei Liu, Yanyan Feng, Hao Zhou, Zhenzhen Zhang, Yuzi Wu, Jia Wang, Yuan Gan, Ting Yuan, Meng Wu, Xing Xie and Zhixin Feng

Mannan oligosaccharide requires functional ETC and TLR for biological radiation protection to normal cells

Low LET Ionizing radiation is known to alter intracellular redox balance by inducing free radical generation, which may cause oxidative modification of various cellular biomolecules. The extent of biomolecule-...

Authors: Sweta Sanguri and Damodar Gupta

An easy-to-build and re-usable microfluidic system for live-cell imaging

Real-time monitoring of cellular responses to dynamic changes in their environment or to specific treatments has become central to cell biology. However, when coupled to live-cell imaging, such strategies are ...

Authors: Julien Babic, Laurent Griscom, Jeremy Cramer and Damien Coudreuse

A comparison of LKB1/AMPK/mTOR metabolic axis response to global ischaemia in brain, heart, liver and kidney in a rat model of cardiac arrest

Cellular energy failure in high metabolic rate organs is one of the underlying causes for many disorders such as neurodegenerative diseases, cardiomyopathies, liver and renal failures. In the past decade, nume...

Authors: Shohreh Majd, John H. T. Power, Timothy K. Chataway and Hugh J. M. Grantham

The C-terminus of the oncoprotein TGAT is necessary for plasma membrane association and efficient RhoA-mediated signaling

Rho guanine exchange factors (RhoGEFs) control cellular processes such as migration, adhesion and proliferation. Alternative splicing of the RhoGEF Trio produces TGAT. The RhoGEF TGAT is an oncoprotein with co...

Authors: J. van Unen, D. Botman, T. Yin, Y. I. Wu, M. A. Hink, T. W. J. Gadella Jr, M. Postma and J. Goedhart

Overexpression of Contactin 1 promotes growth, migration and invasion in Hs578T breast cancer cells

Contactin1 (CNTN1) has been shown to play an important role in the invasion and metastasis of several tumors; however, the role of CNTN1 in breast cancer has not been fully studied. The purpose of this study i...

Authors: Nan Chen, Sai He, Jie Geng, Zhang-Jun Song, Pi-Hua Han, Juan Qin, Zheng Zhao, Yong-Chun Song, Hu-Xia Wang and Cheng-Xue Dang

Foreskin-derived mesenchymal stromal cells with aldehyde dehydrogenase activity: isolation and gene profiling

Mesenchymal stromal cells (MSCs) become an attractive research topic because of their crucial roles in tissue repair and regenerative medicine. Foreskin is considered as a valuable tissue source containing imm...

Authors: Mehdi Najar, Emerence Crompot, Leo A. van Grunsven, Laurent Dollé and Laurence Lagneaux

Role of the HIV-1 envelope transmembrane domain in intracellular sorting

The envelope protein of lentiviruses are type I transmembrane proteins, and their transmembrane domain contains conserved potentially charged residues. This highly unusual feature would be expected to cause en...

Authors: Jackie Perrin, Aurélie Bary, Alexandre Vernay and Pierre Cosson

Separation of functionally divergent muscle precursor cell populations from porcine juvenile muscles by discontinuous Percoll density gradient centrifugation

Satellite cells (SC) and their descendants, muscle precursor cells (MPC), play a key role in postnatal muscle development, regeneration, and plasticity. Several studies have provided evidence that SC and MPC r...

Authors: Claudia Miersch, Katja Stange and Monika Röntgen

MicroRNA-17-5p aggravates lipopolysaccharide-induced injury in nasal epithelial cells by targeting Smad7

Globally, rhinitis is one of the most common chronic disorders. Despite availability of drugs to manage the symptomatology of rhinitis, researchers still focus on identification of novel molecular targets for ...

Authors: Nan Huang, Wenjing Li, Xiaolong Wang and Shanshan Qi

Mechanically tuned 3 dimensional hydrogels support human mammary fibroblast growth and viability

Carcinoma associated fibroblasts (CAFs or myofibroblasts) are activated fibroblasts which participate in breast tumor growth, angiogenesis, invasion, metastasis and therapy resistance. As such, recent efforts ...

Authors: Kathryn Woods, Catlyn Thigpen, Jennifer Peyling Wang, Hana Park and Abigail Hielscher

Molecular targets and signaling pathways regulated by nuclear translocation of syndecan-1

The cell-surface heparan sulfate proteoglycan syndecan-1 is important for tumor cell proliferation, migration, and cell cycle regulation in a broad spectrum of malignancies. Syndecan-1, however, also transloca...

Authors: Tünde Szatmári, Filip Mundt, Ashish Kumar-Singh, Lena Möbus, Rita Ötvös, Anders Hjerpe and Katalin Dobra

A novel interaction between kinase activities in regulation of cilia formation

The primary cilium is an extension of the cell membrane that encloses a microtubule-based axoneme. Primary cilia are essential for transmission of environmental cues that determine cell fate. Disruption of pri...

Authors: Nicole DeVaul, Katerina Koloustroubis, Rong Wang and Ann O. Sperry

Reduction of pluripotent gene expression in murine embryonic stem cells exposed to mechanical loading or Cyclo RGD peptide

Self-renewal and differentiation of embryonic stem cells (ESCs) is directed by biological and/or physical cues that regulate multiple signaling cascades. We have previously shown that mESCs seeded in a type I ...

Authors: Olesja Hazenbiller, Neil A. Duncan and Roman J. Krawetz

Small molecule modulation of splicing factor expression is associated with rescue from cellular senescence

Altered expression of mRNA splicing factors occurs with ageing in vivo and is thought to be an ageing mechanism. The accumulation of senescent cells also occurs in vivo with advancing age and causes much degen...

Authors: Eva Latorre, Vishal C. Birar, Angela N. Sheerin, J. Charles C. Jeynes, Amy Hooper, Helen R. Dawe, David Melzer, Lynne S. Cox, Richard G. A. Faragher, Elizabeth L. Ostler and Lorna W. Harries

Coral cell separation and isolation by fluorescence-activated cell sorting (FACS)

Generalized methods for understanding the cell biology of non-model species are quite rare, yet very much needed. In order to address this issue, we have modified a technique traditionally used in the biomedic...

Authors: Benyamin Rosental, Zhanna Kozhekbaeva, Nathaniel Fernhoff, Jonathan M. Tsai and Nikki Traylor-Knowles

Honokiol improved chondrogenesis and suppressed inflammation in human umbilical cord derived mesenchymal stem cells via blocking nuclear factor-κB pathway

Cartilage degradation is the significant pathological process in osteoarthritis (OA). Inflammatory cytokines, such as interleukin-1β (IL-1β), activate various downstream mediators contributing to OA pathology....

Authors: Hao Wu, Zhanhai Yin, Ling Wang, Feng Li and Yusheng Qiu

EphA receptors and ephrin-A ligands are upregulated by monocytic differentiation/maturation and promote cell adhesion and protrusion formation in HL60 monocytes

Eph signaling is known to induce contrasting cell behaviors such as promoting and inhibiting cell adhesion/spreading by altering F-actin organization and influencing integrin activities. We have previously dem...

Authors: Midori Mukai, Norihiko Suruga, Noritaka Saeki and Kazushige Ogawa

Erratum to: Cladosporol a triggers apoptosis sensitivity by ROS-mediated autophagic flux in human breast cancer cells

Authors: Mytre Koul, Ashok Kumar, Ramesh Deshidi, Vishal Sharma, Rachna D. Singh, Jasvinder Singh, Parduman Raj Sharma, Bhahwal Ali Shah, Sundeep Jaglan and Shashank Singh