Articles

Cyclic AMP signaling in Dictyostelium promotes the translocation of the copine family of calcium-binding proteins to the plasma membrane

Copines are calcium-dependent phospholipid-binding proteins found in many eukaryotic organisms and are thought to be involved in signaling pathways that regulate a wide variety of cellular processes. Copines a...

Authors: April N. Ilacqua, Janet E. Price, Bria N. Graham, Matthew J. Buccilli, Dexter R. McKellar and Cynthia K. Damer

mTOR and ROS regulation by anethole on adipogenic differentiation in human mesenchymal stem cells

Adipocyte differentiation of human mesenchymal stem cells (hMSCs) is dependent on mitochondrial metabolism and reactive oxygen species (ROS) to initiate adipocyte differentiation. Although anethole has been kn...

Authors: Yun-Hee Rhee, Jeong Hwan Moon, Ji-Hun Mo, Tiffany Pham and Phil-Sang Chung

Caspase-independent cell death does not elicit a proliferative response in melanoma cancer cells

Apoptosis, the most well-known type of programmed cell death, can induce in a paracrine manner a proliferative response in neighboring surviving cells called apoptosis-induced proliferation (AiP). While having...

Authors: Ahlima Roumane, Kevin Berthenet, Chaïmaa El Fassi and Gabriel Ichim

Establishment and comparison of air-liquid interface culture systems for primary and immortalized swine tracheal epithelial cells

Air-liquid interface (Ali) systems allow the establishment of a culture environment more representative of that in vivo than other culture systems. They are useful for performing mechanistic studies of respira...

Authors: Haiyan Wang, Lina He, Beibei Liu, Yanyan Feng, Hao Zhou, Zhenzhen Zhang, Yuzi Wu, Jia Wang, Yuan Gan, Ting Yuan, Meng Wu, Xing Xie and Zhixin Feng

Mannan oligosaccharide requires functional ETC and TLR for biological radiation protection to normal cells

Low LET Ionizing radiation is known to alter intracellular redox balance by inducing free radical generation, which may cause oxidative modification of various cellular biomolecules. The extent of biomolecule-...

Authors: Sweta Sanguri and Damodar Gupta

An easy-to-build and re-usable microfluidic system for live-cell imaging

Real-time monitoring of cellular responses to dynamic changes in their environment or to specific treatments has become central to cell biology. However, when coupled to live-cell imaging, such strategies are ...

Authors: Julien Babic, Laurent Griscom, Jeremy Cramer and Damien Coudreuse

A comparison of LKB1/AMPK/mTOR metabolic axis response to global ischaemia in brain, heart, liver and kidney in a rat model of cardiac arrest

Cellular energy failure in high metabolic rate organs is one of the underlying causes for many disorders such as neurodegenerative diseases, cardiomyopathies, liver and renal failures. In the past decade, nume...

Authors: Shohreh Majd, John H. T. Power, Timothy K. Chataway and Hugh J. M. Grantham

The C-terminus of the oncoprotein TGAT is necessary for plasma membrane association and efficient RhoA-mediated signaling

Rho guanine exchange factors (RhoGEFs) control cellular processes such as migration, adhesion and proliferation. Alternative splicing of the RhoGEF Trio produces TGAT. The RhoGEF TGAT is an oncoprotein with co...

Authors: J. van Unen, D. Botman, T. Yin, Y. I. Wu, M. A. Hink, T. W. J. Gadella Jr, M. Postma and J. Goedhart

Overexpression of Contactin 1 promotes growth, migration and invasion in Hs578T breast cancer cells

Contactin1 (CNTN1) has been shown to play an important role in the invasion and metastasis of several tumors; however, the role of CNTN1 in breast cancer has not been fully studied. The purpose of this study i...

Authors: Nan Chen, Sai He, Jie Geng, Zhang-Jun Song, Pi-Hua Han, Juan Qin, Zheng Zhao, Yong-Chun Song, Hu-Xia Wang and Cheng-Xue Dang

Foreskin-derived mesenchymal stromal cells with aldehyde dehydrogenase activity: isolation and gene profiling

Mesenchymal stromal cells (MSCs) become an attractive research topic because of their crucial roles in tissue repair and regenerative medicine. Foreskin is considered as a valuable tissue source containing imm...

Authors: Mehdi Najar, Emerence Crompot, Leo A. van Grunsven, Laurent Dollé and Laurence Lagneaux

Role of the HIV-1 envelope transmembrane domain in intracellular sorting

The envelope protein of lentiviruses are type I transmembrane proteins, and their transmembrane domain contains conserved potentially charged residues. This highly unusual feature would be expected to cause en...

Authors: Jackie Perrin, Aurélie Bary, Alexandre Vernay and Pierre Cosson

Separation of functionally divergent muscle precursor cell populations from porcine juvenile muscles by discontinuous Percoll density gradient centrifugation

Satellite cells (SC) and their descendants, muscle precursor cells (MPC), play a key role in postnatal muscle development, regeneration, and plasticity. Several studies have provided evidence that SC and MPC r...

Authors: Claudia Miersch, Katja Stange and Monika Röntgen

MicroRNA-17-5p aggravates lipopolysaccharide-induced injury in nasal epithelial cells by targeting Smad7

Globally, rhinitis is one of the most common chronic disorders. Despite availability of drugs to manage the symptomatology of rhinitis, researchers still focus on identification of novel molecular targets for ...

Authors: Nan Huang, Wenjing Li, Xiaolong Wang and Shanshan Qi

Mechanically tuned 3 dimensional hydrogels support human mammary fibroblast growth and viability

Carcinoma associated fibroblasts (CAFs or myofibroblasts) are activated fibroblasts which participate in breast tumor growth, angiogenesis, invasion, metastasis and therapy resistance. As such, recent efforts ...

Authors: Kathryn Woods, Catlyn Thigpen, Jennifer Peyling Wang, Hana Park and Abigail Hielscher

Molecular targets and signaling pathways regulated by nuclear translocation of syndecan-1

The cell-surface heparan sulfate proteoglycan syndecan-1 is important for tumor cell proliferation, migration, and cell cycle regulation in a broad spectrum of malignancies. Syndecan-1, however, also transloca...

Authors: Tünde Szatmári, Filip Mundt, Ashish Kumar-Singh, Lena Möbus, Rita Ötvös, Anders Hjerpe and Katalin Dobra

A novel interaction between kinase activities in regulation of cilia formation

The primary cilium is an extension of the cell membrane that encloses a microtubule-based axoneme. Primary cilia are essential for transmission of environmental cues that determine cell fate. Disruption of pri...

Authors: Nicole DeVaul, Katerina Koloustroubis, Rong Wang and Ann O. Sperry

Reduction of pluripotent gene expression in murine embryonic stem cells exposed to mechanical loading or Cyclo RGD peptide

Self-renewal and differentiation of embryonic stem cells (ESCs) is directed by biological and/or physical cues that regulate multiple signaling cascades. We have previously shown that mESCs seeded in a type I ...

Authors: Olesja Hazenbiller, Neil A. Duncan and Roman J. Krawetz

Small molecule modulation of splicing factor expression is associated with rescue from cellular senescence

Altered expression of mRNA splicing factors occurs with ageing in vivo and is thought to be an ageing mechanism. The accumulation of senescent cells also occurs in vivo with advancing age and causes much degen...

Authors: Eva Latorre, Vishal C. Birar, Angela N. Sheerin, J. Charles C. Jeynes, Amy Hooper, Helen R. Dawe, David Melzer, Lynne S. Cox, Richard G. A. Faragher, Elizabeth L. Ostler and Lorna W. Harries

Coral cell separation and isolation by fluorescence-activated cell sorting (FACS)

Generalized methods for understanding the cell biology of non-model species are quite rare, yet very much needed. In order to address this issue, we have modified a technique traditionally used in the biomedic...

Authors: Benyamin Rosental, Zhanna Kozhekbaeva, Nathaniel Fernhoff, Jonathan M. Tsai and Nikki Traylor-Knowles

Honokiol improved chondrogenesis and suppressed inflammation in human umbilical cord derived mesenchymal stem cells via blocking nuclear factor-κB pathway

Cartilage degradation is the significant pathological process in osteoarthritis (OA). Inflammatory cytokines, such as interleukin-1β (IL-1β), activate various downstream mediators contributing to OA pathology....

Authors: Hao Wu, Zhanhai Yin, Ling Wang, Feng Li and Yusheng Qiu

EphA receptors and ephrin-A ligands are upregulated by monocytic differentiation/maturation and promote cell adhesion and protrusion formation in HL60 monocytes

Eph signaling is known to induce contrasting cell behaviors such as promoting and inhibiting cell adhesion/spreading by altering F-actin organization and influencing integrin activities. We have previously dem...

Authors: Midori Mukai, Norihiko Suruga, Noritaka Saeki and Kazushige Ogawa

Erratum to: Cladosporol a triggers apoptosis sensitivity by ROS-mediated autophagic flux in human breast cancer cells

Authors: Mytre Koul, Ashok Kumar, Ramesh Deshidi, Vishal Sharma, Rachna D. Singh, Jasvinder Singh, Parduman Raj Sharma, Bhahwal Ali Shah, Sundeep Jaglan and Shashank Singh

Cladosporol A triggers apoptosis sensitivity by ROS-mediated autophagic flux in human breast cancer cells

Endophytes have proven to be an invaluable resource of chemically diverse secondary metabolites that act as excellent lead compounds for anticancer drug discovery. Here we report the promising cytotoxic effect...

Authors: Mytre Koul, Ashok Kumar, Ramesh Deshidi, Vishal Sharma, Rachna D. Singh, Jasvinder Singh, Parduman Raj Sharma, Bhahwal Ali Shah, Sundeep Jaglan and Shashank Singh

The M-phase specific hyperphosphorylation of Staufen2 involved the cyclin-dependent kinase CDK1

Staufen2 (STAU2) is an RNA-binding protein involved in the post-transcriptional regulation of gene expression. This protein was shown to be required for organ formation and cell differentiation. Although STAU2...

Authors: Rémy Beaujois, Elizabeth Ottoni, Xin Zhang, Christina Gagnon, Sami HSine, Stéphanie Mollet, Wildriss Viranaicken and Luc DesGroseillers

External magnetic field promotes homing of magnetized stem cells following subcutaneous injection

Mesenchymal stem cells (MSCs) are multipotent stromal cells that have the ability to self-renew and migrate to sites of pathology. In vivo tracking of MSCs provides insights into both, the underlying mechanism...

Authors: Yu Meng, Changzhen Shi, Bo Hu, Jian Gong, Xing Zhong, Xueyin Lin, Xinju Zhang, Jun Liu, Cong Liu and Hao Xu

Adenylyl cyclase mRNA localizes to the posterior of polarized DICTYOSTELIUM cells during chemotaxis

In Dictyostelium discoideum, vesicular transport of the adenylyl cyclase A (ACA) to the posterior of polarized cells is essential to relay exogenous 3′,5′-cyclic

Authors: Satarupa Das, Joshua M. Parker, Can Guven, Weiye Wang, Paul W. Kriebel, Wolfgang Losert, Daniel R. Larson and Carole A. Parent

ULK1 phosphorylates Sec23A and mediates autophagy-induced inhibition of ER-to-Golgi traffic

Autophagy is an inducible autodigestive process that allows cells to recycle proteins and other materials for survival during stress and nutrient deprived conditions. The kinase ULK1 is required to activate th...

Authors: Wenjia Gan, Caiyun Zhang, Ka Yu Siu, Ayano Satoh, Julian A. Tanner and Sidney Yu

Dental pulp pluripotent-like stem cells (DPPSC), a new stem cell population with chromosomal stability and osteogenic capacity for biomaterials evaluation

Biomaterials are widely used to regenerate or substitute bone tissue. In order to evaluate their potential use for clinical applications, these need to be tested and evaluated in vitro with cell culture models...

Authors: Raquel Núñez-Toldrà, Ester Martínez-Sarrà, Carlos Gil-Recio, Miguel Ángel Carrasco, Ashraf Al Madhoun, Sheyla Montori and Maher Atari

Loss of pericyte smoothened activity in mice with genetic deficiency of leptin

Obesity is associated with multiple diseases, but it is unclear how obesity promotes progressive tissue damage. Recovery from injury requires repair, an energy-expensive process that is coupled to energy avail...

Authors: Guanhua Xie, Marzena Swiderska-Syn, Mark L. Jewell, Mariana Verdelho Machado, Gregory A. Michelotti, Richard T. Premont and Anna Mae Diehl

Intermittent parathyroid hormone (PTH) promotes cementogenesis and alleviates the catabolic effects of mechanical strain in cementoblasts

External root resorption, commonly starting from cementum, is a severe side effect of orthodontic treatment. In this pathological process and repairing course followed, cementoblasts play a significant role. P...

Authors: Yuyu Li, Zhiai Hu, Chenchen Zhou, Yang Xu, Li Huang, Xin Wang and Shujuan Zou

Stress induced nuclear granules form in response to accumulation of misfolded proteins in Caenorhabditis elegans

Environmental stress can affect the viability or fecundity of an organism. Environmental stressors may affect the genome or the proteome and can cause cellular distress by contributing to protein damage or mis...

Authors: Katherine M. Sampuda, Mason Riley and Lynn Boyd

Impact of the MDM2 splice-variants MDM2-A, MDM2-B and MDM2-C on cytotoxic stress response in breast cancer cells

The murine double minute 2 (MDM2) is an oncogene and a negative regulator of the tumor suppressor protein p53. MDM2 is known to be amplified in numerous human cancers, and upregulation of MDM2 is considered to be...

Authors: Johanna Huun, Liv B. Gansmo, Bård Mannsåker, Gjertrud Titlestad Iversen, Jan Inge Øvrebø, Per E. Lønning and Stian Knappskog

Testis-specific ATP synthase peripheral stalk subunits required for tissue-specific mitochondrial morphogenesis in Drosophila

In Drosophila early post-meiotic spermatids, mitochondria undergo dramatic shaping into the Nebenkern, a spherical body with complex internal structure that contains two interwrapped giant mitochondrial derivativ...

Authors: Eric M. Sawyer, Elizabeth C. Brunner, Yihharn Hwang, Lauren E. Ivey, Olivia Brown, Megan Bannon, Dennis Akrobetu, Kelsey E. Sheaffer, Oshauna Morgan, Conroy O. Field, Nishita Suresh, M. Grace Gordon, E. Taylor Gunnell, Lindsay A. Regruto, Cricket G. Wood, Margaret T. Fuller…

Mesenchymal stem cells and myoblast differentiation under HGF and IGF-1 stimulation for 3D skeletal muscle tissue engineering

Volumetric muscle loss caused by trauma or after tumour surgery exceeds the natural regeneration capacity of skeletal muscle. Hence, the future goal of tissue engineering (TE) is the replacement and repair of ...

Authors: R. Witt, A. Weigand, A. M. Boos, A. Cai, D. Dippold, A. R. Boccaccini, D. W. Schubert, M. Hardt, C. Lange, A. Arkudas, R. E. Horch and J. P. Beier

Hinokitiol induces DNA demethylation via DNMT1 and UHRF1 inhibition in colon cancer cells

DNA hypermethylation is a key epigenetic mechanism for the silencing of many genes in cancer. Hinokitiol, a tropolone-related natural compound, is known to induce apoptosis and cell cycle arrest and has anti-i...

Authors: Jung Seon Seo, Young Ha Choi, Ji Wook Moon, Hyeon Soo Kim and Sun-Hwa Park

Bmp-12 activates tenogenic pathway in human adipose stem cells and affects their immunomodulatory and secretory properties

Cell-based therapy is a treatment method in tendon injuries. Bone morphogenic protein 12 (BMP-12) possesses tenogenic activity and was proposed as a differentiating factor for stem cells directed to transplant...

Authors: Weronika Zarychta-Wiśniewska, Anna Burdzinska, Agnieszka Kulesza, Kamila Gala, Beata Kaleta, Katarzyna Zielniok, Katarzyna Siennicka, Marek Sabat and Leszek Paczek

Variation in human dental pulp stem cell ageing profiles reflect contrasting proliferative and regenerative capabilities

Dental pulp stem cells (DPSCs) are increasingly being recognized as a viable cell source for regenerative medicine. Although significant variations in their ex vivo expansion are well-established, DPSC prolife...

Authors: Amr Alraies, Nadia Y. A. Alaidaroos, Rachel J. Waddington, Ryan Moseley and Alastair J. Sloan

Nuclear and structural dynamics during the establishment of a specialized effector-secreting cell by Magnaporthe oryzae in living rice cells

To cause an economically important blast disease on rice, the filamentous fungus Magnaporthe oryzae forms a specialized infection structure, called an appressorium, to penetrate host cells. Once inside host cells...

Authors: Emma N. Shipman, Kiersun Jones, Cory B. Jenkinson, Dong Won Kim, Jie Zhu and Chang Hyun Khang

Release of endothelial cell associated VEGFR2 during TGF-β modulated angiogenesis in vitro

Sprouting angiogenesis requires vascular endothelial proliferation, migration and morphogenesis. The process is regulated by soluble factors, principally vascular endothelial growth factor (VEGF), and via bidi...

Authors: M. Jarad, E. A. Kuczynski, J. Morrison, A. M. Viloria-Petit and B. L. Coomber

miR-27b inhibits fibroblast activation via targeting TGFβ signaling pathway

MicroRNAs are a group of small RNAs that regulate gene expression at the posttranscriptional level. They regulate almost every aspect of cellular processes. In this study, we investigated whether miR-27b regul...

Authors: Xiangming Zeng, Chaoqun Huang, Lakmini Senavirathna, Pengcheng Wang and Lin Liu

Intermittent hypoxia causes NOX2-dependent remodeling of atrial connexins

Obstructive sleep apnea has been linked to the development of heart disease and arrhythmias, including atrial fibrillation. Since altered conduction through gap junction channels can contribute to the pathogen...

Authors: Joanna Gemel, Zihan Su, Alex Gileles-Hillel, Abdelnaby Khalyfa, David Gozal and Eric C. Beyer

Focus on lens connexins

The lens is an avascular organ composed of an anterior epithelial cell layer and fiber cells that form the bulk of the organ. The lens expresses connexin43 (Cx43), connexin46 (Cx46) and connexin50 (Cx50). Epit...

Authors: Viviana M. Berthoud and Anaclet Ngezahayo

Accessing gap-junction channel structure-function relationships through molecular modeling and simulations

Gap junction channels (GJCs) are massive protein channels connecting the cytoplasm of adjacent cells. These channels allow intercellular transfer of molecules up to ~1 kDa, including water, ions and other meta...

Authors: F. Villanelo, Y. Escalona, C. Pareja-Barrueto, J. A. Garate, I. M. Skerrett and T. Perez-Acle

Gap junctions and hemichannels: communicating cell death in neurodevelopment and disease

Gap junctions are unique membrane channels that play a significant role in intercellular communication in the developing and mature central nervous system (CNS). These channels are composed of connexin protein...

Authors: Andrei B. Belousov, Joseph D. Fontes, Moises Freitas-Andrade and Christian C. Naus

Cardiac Cx43, Cx40 and Cx45 co-assembling: involvement of connexins epitopes in formation of hemichannels and Gap junction channels

This review comes after the International Gap Junction Conference (IGJC 2015) and describes the current knowledge on the function of the specific motifs of connexins in the regulation of the formation of gap j...

Authors: Thomas Desplantez

Pannexin channel and connexin hemichannel expression in vascular function and inflammation

Control of blood flow distribution and tissue homeostasis depend on the tight regulation of and coordination between the microvascular network and circulating blood cells. Channels formed by connexins or panne...

Authors: Daniela Begandt, Miranda E Good, Alex S. Keller, Leon J. DeLalio, Carol Rowley, Brant E. Isakson and Xavier F. Figueroa

Nuclear envelope structural proteins facilitate nuclear shape changes accompanying embryonic differentiation and fidelity of gene expression

Nuclear size and shape are specific to a cell type, function, and location, and can serve as indicators of disease and development. We previously found that lamin A/C and associated nuclear envelope structural...

Authors: Elizabeth R. Smith, Yue Meng, Robert Moore, Jeffrey D. Tse, Arn G. Xu and Xiang-Xi Xu

Erratum to: Nuclear envelope structural defect underlies the main cause of aneuploidy in ovarian carcinogenesis

Authors: Callinice D. Capo-chichi, Toni M. Yeasky, Elizabeth R. Smith and Xiang-Xi Xu

Hierarchical imaging: a new concept for targeted imaging of large volumes from cells to tissues

Imaging large volumes such as entire cells or small model organisms at nanoscale resolution seemed an unrealistic, rather tedious task so far. Now, technical advances have lead to several electron microscopy (...

Authors: Irene Wacker, Waldemar Spomer, Andreas Hofmann, Marlene Thaler, Stefan Hillmer, Ulrich Gengenbach and Rasmus R. Schröder

Nuclear envelope structural defect underlies the main cause of aneuploidy in ovarian carcinogenesis

The Cancer Atlas project has shown that p53 is the only commonly (96 %) mutated gene found in high-grade serous epithelial ovarian cancer, the major histological subtype. Another general genetic change is exte...

Authors: Callinice D. Capo-chichi, Toni M. Yeasky, Elizabeth R. Smith and Xiang-Xi Xu