Figure 1From: Sec61β, a subunit of the Sec61 protein translocation channel at the Endoplasmic Reticulum, is involved in the transport of Gurken to the plasma membrane.Sec61β protein and Gurken mRNA in ovaries from wild type and sec61βP1germline clones. A) Ovaries were dissected from flies and lysed in buffer containing 2% SDS. Western blotting was performed using Sec61β antibody. A cross-reactive band serves as a loading control. B) Schematic representation of a stage 9–10 egg chamber with the oocyte abutting the 15 nurse cells and surrounded by a layer of somatic follicle cells. The dorsal/anterior corner is depicted in dark red. C-D) Drosophila egg chambers were processed for gurken RNA in-situ hybridization in wild type oocytes, C) and in oocytes mutant for Sec61β, D). n represents the oocyte nucleus.Back to article page