Figure 1

VN173 and VC155 cassettes for PCR-mediated gene targeting in fission yeast. Central part: schematic of cassette region of pFA6a-based plasmids showing key features, including restriction sites (Pac I, Asc I, Bgl II, Pme I) used for cloning. Abbreviations: TA (S. cerevisiae ADH1 transcriptional terminator), PT (Ashbya gossypii TEF promoter), TT (Ashbya gossypii TEF transcriptional terminator). Upper and lower parts: schematic of VN173 and VC155 Venus YFP fragments. Linker regions are shown (18 and 25 amino acids for VN173 and VC155 respectively).