Figure 3

Quantitative differentiation of mMSCs and GFPTgMSCs along osteogenic and adipogenic lineages. A. Cells were incubated in CEM and then transferred to osteogenic media for 14 days. The cells were then stained with Alizarin Red and de-stained with 10% cetylpyridinium chloride. Optical density (OD) was measured at 560 nm and normalized to protein content. Graphs represent the ratio of normalized OD of differentiated cells and normalized OD of control cells. B. Cells were incubated in CEM and then transferred to adipogenic media for 14 days. The cells were then stained with Oil Red O and de-stained with isopropanol. OD was measured at 520 nm and normalized to protein content. Graphs represent the ratio of normalized OD of differentiated cells and normalized OD of control cells.