Isolation and characterization of a spontaneously immortalized bovine retinal pigmented epithelial cell line

Table 1 Primers used for the reverse transcriptase PCR

Gene		Primer Sequence	Length	Tm (oC)	Product size
CRALBP	forward	AGA ACT CTC CAG CTT CTA CCA GGA	24 bp	60.2	301 bp
	reverse	AGC TTG GGA GGA GGA TGA CAT CTG	24 bp	59.8
β-Actin	forward	TCA TGA TCG AGA CCT TCA ACA CCC	24 bp	60.3	288 bp
	reverse	AGC AGA GCT TCT CCT TGA TGT CAC	24 bp	60.1
RPE-65	forward	CCA GGG CTG CTG AGG TGG TTA ACA	24 bp	60.0	278 bp
	reverse	GTG CCT GTC TCA CGA AGT ACG ATT	24 bp	60.1
Vimentin	forward	ATG AAG GAA GAG ATG GCT CGT CAC	24 bp	59.9	173 bp
	reverse	TCC AGA TTG GTT TCC CTC AGG TTC	24 bp	60.0

Primer sequences, lengths, melting temperatures and product sizes have been included used in the identification of RPE-specific genes and control genes for primary RPE cells and tRPE cell cultures.

ISSN: 2661-8850