Figure 4

Differences in the proliferation rate between the undifferentiated I3, I6 and BG01V cells. All three cell lines were maintained on the MEF for 4 days post-passaging under the same culture conditions. A four hour BrdU pulse shows significant differences in the proliferation index which is defined as the percentage of BrdU+ nuclei among the total number of propidium iodide (PI)+ cells.(A-I) Images of the three cell line colonies (A, D, G) immunostained for BrdU incorporation (C, F, I) and counterstained with PI (B, E, H). Bars in A, D and G = 100 μm. (J) Bar plot summarizing the differences in diameters of the colonies between the three cell lines. Values are expressed as a percent of the total number of cells (mean ± SEM; BG01V- 554 ± 187 μm, I3 – 184 ± 75 μm, and I6 – 488 ± 165 μm. Sizes of colonies derived from the BG01V or I6 cells are significantly greater than those derived from the I3 cells. ** p < 0.01. (K) Bar plot summarizes the differences in the BrdU incorporation levels between three cell lines. The BG01V cells maintained on MEF at 4 days post-passaging exhibit the highest BrdU incorporation levels and the I3 exhibited the lowest levels. Values are expressed as the ratio of BrdU+ cells to the total number of cells (PI stained) (mean ± SEM). Statistical differences for colony diameters or BrdU/PI % between the I6 or BG01V and the I3 cells are significant * p < 0.05.