Figure 3

SLMAP localizes to the ER and its associations are microtubule dependent. (A), COS7 cells were stained with anti-SLMAP (A; a) and anti-calnexin (A; b) and merged image (A; c). (B), COS7 cells were transfected with 6Myc-SLMAP-TA1 (B; a, b, d, e) and 6Myc-SLMAPΔTM (B; c, f) and stained with Anti-SLMAP (B; a-c) and anti-α-tubulin (B; d-f). Cells treated with nocodazole (B; b, c, e, f). (C) COS7 cells transfected with either 6Myc-SLMAP-TA1 (C; a, b, e, f) or 6Myc-SLMAP-TA2 (C; c, d, g, h) and stained with phalloidin (C; e-h) and anti-Myc (a-d). Treatment with cytochalasin D (C; f, h) did not alter the distribution of the 6Myc-SLMAP-TA1 (C; b) or 6Myc-SLMAP-TA2 (C; d). Scale bar = 10 μm.