Figure 3

Rb interaction with mitochondria. Rb interaction with mitochondria: A. [³⁵S]Met-Rb, -Luciferase and -Bax proteins produced in vitro were added to purified rat liver mitochondria and then the mitochondria-bound protein fraction (M1) was separated from the non-mitochondria-bound protein fraction (S1), then subjected to gel electrophoresis and autoradiography using a phosphoimager. In vitro translated proteins, were loaded in parallel as a control (C). B. In vitro translated [³⁵S]Met-Rb, -Luciferase and -Bax were incubated as above and then the mitochondria were alkaline treated (Alk Treat.). Input: translated proteins (C); supernatant containing non-mitochondria-bound proteins (S1); supernatant containing detached proteins after alkaline treatment (S2); mitochondria-bound proteins after alkaline treatment (M2). These data are representative for 3 independent experiments.