Analysis of HMIT expression in rat and human brain. Fluorescence microphotographs of adult male rat coronal sections immunolabelled with anti-HMIT antibody. HMIT-LI distribution in the secondary motor cortex (M2), at approximately +1.20 mm from Bregma at low (A) and high magnification (B); and in the CA3 hippocampal region, approximately -2.80 mm from Bregma (C); the arrowhead indicates a HMIT-positive interneuron; Scale bar = 100 μm (A, C) and 50 μm (B). Microphotographs of formalin-fixed paraffin embedded sections of human brain areas immunolabelled with anti-HMIT antibody. HMIT staining is visible in cell bodies and projections of different regions of the human brain: cerebral cortex (D); hippocampus (E) and thalamus (F), the latter at higher magnification, in which two examples of HMIT positive staining are indicated by the arrowheads. Scale bar = 50 μm (D, E, F).