Figure 1
From: Mechanical strain induces involution-associated events in mammary epithelial cells
Equibiaxial stretching device. (A) Assembled device and its individual components (1–5): 1) Delrin® made cylinder with an inner ring that constitutes the silicone membrane holder; 2) Delrin® made ring with an O-ring that attaches the membrane to the inner holder; 3) Teflon® made indenter ring; 4) Delrin® made flange that pushes down piece #3; 5) Aluminum screw-top, that pushes down piece #4. (B) Diagram (in scale) depicting the procedure to assemble the complete device for performing cell stretching protocols; step 1 indicates positioning of the flexible membrane with attached cells grown in a 60 mm Petri dish; following steps indicate assembly of components shown in (A); (C) Cross-section of the complete device. For each piece, specific dimensions are described in millimeters.