Interaction of SIRT1 with zyxin in mammalian cells. (A, B) Co-immunoprecipitation of SIRT1 and zyxin HEK293T cells transiently transfected with the indicated plasmids and treated with 20 nM of leptomycin B (LMB) for 6 h. Cell lysates were immunoprecipitated with the anti-GFP antibody (A) or anti-HA antibody (B). The immunoprecipitates were immunoblotted with a reciprocal antibody. (C) Endogenous SIRT1 interacts with GFP-zyxin. The lysates of HEK293T cells expressing GFP-zyxin were immunoprecipitated with anti-SIRT1 antibody or mouse IgG. The immunoprecipitates were immunoblotted with anti-GFP antibody.