Figure 4

Changes in [Ca²⁺] _i in response to CCK-8 and effect of ethanol. Time-course of changes in [Ca²⁺]_i in fura-2-loaded mouse pancreatic acinar cells. Cells were stimulated with 1 nM CCK-8 alone (full circles) or in the presence of 50 mM ethanol (EtOH; open circles) in the presence of Ca²⁺ in the extracellular medium. The arrow indicates the time point at which CCK-8 was added to the cells. In the experiments in which its effect was assayed, EtOH was added to the cells 1 minute prior to addition of CCK-8. The traces are typical of 14-17 such independent experiments.