Figure 7
From: Cbl-associated protein is tyrosine phosphorylated by c-Abl and c-Src kinases
Phosphorylation of CAP in SYF cells can be rescued upon expression of active forms of Src. Embryonic fibroblasts from the triple knock-out mouse for Src, Yes and Fyn (SYF cells) were transfected with CAP-EGFP together with various forms of Src kinase and stimulated with vanadate. No Tyr phosphorylation of CAP could be detected in cells expressing only CAP-EGFP or the inactive Src (Dead-Src). Phosphorylation of CAP was rescued upon coexpression of either c-Src or the constitutively active mutant Y527F-Src.