Figure 11
From: The proline-rich domain of tau plays a role in interactions with actin
High speed co-sedimentation assays of tauPRD and F-actin in the presence of NaCl. F-actin (at a constant amount) was incubated with different amounts of tauPRD (panel a) and tau (panel b) in binding buffer with 150 mM NaCl at 37°C for 40 min to mimic physiological ionic strength and then centrifuged (100,000 g, 4°C, 60 min). The pellet and supernatant were electrophoresed on 12% SDS-PAGE gels.