Figure 8
From: The proline-rich domain of tau plays a role in interactions with actin
Low speed co-sedimentation assay of F-actin in the presence of tauPRD. Conditions were the same as those described for Figure 4, except that F-actin (5 μg) from skeletal muscle (used in panels a, b and c) and platelet actin (used in panel d) were incubated with tau at concentrations of 0, 1, 3, 5, 7, 10, and 15 μM (lanes 3 to 9 in each panel) and with tauPRD or tauMTBD at concentrations of 0, 5, 10, 15, 25, 35, and 45 μM (lanes 3 to 9). After sedimentation at 25,000 g, pellets of bundles were electrophoresed on SDS-PAGE gels. Total amount of F-actin (lane 1) and BSA alone (lane 2) were used as controls.