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Figure 1 | BMC Cell Biology

Figure 1

From: Novel interactions of CLN5 support molecular networking between Neuronal Ceroid Lipofuscinosis proteins

Figure 1

Interactions of CLN5 with other NCL proteins. The mouse Cln5-cDNA (coding for aa 26-341) was expressed as GST fusion protein and used to pull down NCL proteins from COS-1 cell lysates. PPT1, CLN3, CLN6-c-myc and CLN8-HA were transiently overexpressed in COS-1 cells and pulled down with GST-mCLN5 and GST vector control. CLN2 and LAMP-1 proteins were endogenous. The bound proteins were immunoblotted and detected with specific antibodies listed in Methods section.

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BMC Molecular and Cell Biology

ISSN: 2661-8850

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