Interactions of CLN5 with other NCL proteins. The mouse Cln5-cDNA (coding for aa 26-341) was expressed as GST fusion protein and used to pull down NCL proteins from COS-1 cell lysates. PPT1, CLN3, CLN6-c-myc and CLN8-HA were transiently overexpressed in COS-1 cells and pulled down with GST-mCLN5 and GST vector control. CLN2 and LAMP-1 proteins were endogenous. The bound proteins were immunoblotted and detected with specific antibodies listed in Methods section.