Consequences of the ER-resident trafficking deficient CLN5 on the localization of CLN2, CLN6 and CLN8 in HeLa cells. HeLa cells were transiently transfected alone with the trafficking deficient CLN5 (CLN5-TD) carrying the intramolecular flag-tag after aa 330 (A-C) or together with wild type CLN6 (D-F) or CLN8 (G-I). The cells were fixed with methanol 48 h post transfection, stained and analyzed by confocal microscopy. The CLN5-TD did not have an effect on the lysosomal localization of endogenous CLN2 (A-C) or on the overexpressed ER resident NCL proteins CLN6 (D-F) and CLN8 (G-I). Scale bar 10 μm.