Figure 4

SYPRO stained gel of co-immunoprecipitated proteins in extracts from HT1080 cells transiently transfected with either of DDIT3 -GFP, FUS-DDIT3 -GFP or GFP constructs. CDK2 antibodies were used for immunoprecipitations. Boxes indicate regions of the gel analyzed by mass spectrometry. Three proteins were enriched in immunoprecipitates from DDIT3 and FUS-DDIT3 expressing cells (region 1: plectin, region 2: myosin, region 3: vimentin). Arrows indicate the differentially immunoprecipitated vimentin bands present in lanes of DDIT3 and FUS-DDIT3 cells but absent in that of GFP cells. These bands migrate slightly slower than the thicker bands inside the same region. The thicker bands were identified as rabbit heavy chain immunoglobulin molecules and these originate from the antibodies used for immunoprecipitation of CDK2 and are present in all three lanes.